Bio-Nano Interfaces and Engineering Applications: Bio-Nano Interfaces: Engineering Applications
Sponsored by: TMS Functional Materials Division, TMS Structural Materials Division, TMS: Biomaterials Committee
Program Organizers: Candan Tamerler, University of Kansas; John Nychka, University of Alberta; Kalpana Katti, North Dakota State University; Terry Lowe, Colorado School of Mines
Tuesday 2:00 PM
February 28, 2017
Room: Pacific 21
Location: Marriott Marquis Hotel
Session Chair: Po-Yu Chen, National Tsing Hua University; Mohan Edirisinghe, University College London
2:00 PM Keynote
Novel Gyratory Processes to Manufacture Bionanointerfaces: Mohan Edirisinghe1; 1University College London
This keynote paper will review our recent work on the invention of gyratory manufacturing processes for forming biointerfaces. At the forefront is the one-pot gyratory method where simultaneously applying gas under pressure and rotating the system rapidly, pressurised gyration (PG) rapidly extrudes a solution of the polymer under ambient temperatures to produce nanofibres. Pressure can be replaced by control of in-flow of material, infusion gyration (IG) and both process can be combined (PCIG). PG, IG and PCIG can also be carried out at elevated temperatures to facilitate solvent-free fibre production. Our current design includes new auto in-situ collection gyratory systems with increasing the pot diameter/height/number of outlet holes to increase rotating speeds, working pressures and in-flow. Unlike electrospinning, our gyratory methods do not require any high voltage electric field and any nozzles/needles. Aligned or separate fibres can be obtained by regulating the rotation speed, and the process is also scalable.
Bio-inspired Syntheses of Self-cleaning Coatings and Oil-water Separation Interfaces by Atmospheric Pressure Plasma and Freeze Casting Techniques: Po-Yu Chen1; Ching-Yu Yang1; Yu-Hsiang Lo1; 1National Tsing Hua University
Biological materials, which have risen from hundreds of million years of evolution, have offered inspirations in the designs of novel materials that fulfill various functional purposes. In this study, we developed a novel facile methodology for fabricating multi-functional self-cleaning surfaces by atmospheric pressure plasma technique and surface modification. The synthesized coating displayed high transparency, high temperature durability and mechanical stability. The performances of self-cleaning and oil-water separation were successfully demonstrated. Moreover, we discovered that diatomite, a silica-based, nano-porous bio-material, serves as a good candidate for improving the performance of oil-water separation. Through the freeze casting technique, the synthesized hierarchically porous plate exhibited underwater superoleophobicity. Furthermore, the plate exhibits excellent environmental stability under a series of harsh environments, including strong acidic, brine, and hot water. To recapitulate, we report two cost-effective, eco-friendly, feasible, and robust methods for synthesizing multifunctional surfaces and interfaces which can be widely applied in various fields.
3:00 PM Cancelled
Biomimetic Lipid Bilayers in Biosensing Applications: Abdulhalim Kilic1; Majid Jadidi1; Hakan Ozgur Ozer1; Fatma Nese Kok1; 1Istanbul Technical University
Biological processes involving membrane proteins range from cell-cell / cell-surface interactions to hormonal regulation and these proteins are the targets of various drugs and toxins. Their hydrophobic structure differs from that of water soluble globular proteins and necessitates a cell membrane mimicking system to study their interactions with other molecules in vitro conditions. Solid-supported lipid bilayers are one of the most widely used platforms in this area since they allow functional integration of membrane proteins and can be characterized by surface sensitive techniques such as Quartz Crystal Microbalance (QCM), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy. QCM and SPR offer the advantage of label-free detection and allow the real time monitoring of binding events enabling the determination of kinetic parameters. These biomimetic lipid bilayers can therefore be used as highly sensitive biosensing platforms to study basic biological interactions or in practical applications like drug screening.
Peptide Enabled Addressable Immobilization of Kinetically Matched Fusion Enzymes in Membrane Flow Bioreactors: Deniz Yucesoy1; Susrut Akkineni1; Bruce Hinds1; Candan Tamerler1; Mehmet Sarikaya1; 1University of Washington
Biomimetic reconstruction of metabolic pathways by incorporating multiple enzymes and relevant cofactors in confined environments holds tremendous promise for sustainable green synthesis of fine chemicals and developing efficient biomolecular devices. The major challenge in designing such systems is to control spatial organization of multiple biological components in sequentially with high spatial precision. GEPI tags (genetically engineered peptides for inorganic solids) have emerged as novel surface functionalization moieties because of their exquisite molecular recognition properties. These solid-binding peptides are ideal candidate as a molecular linkers to immobilize biomolecules on variety of solid surfaces. Here, we use GEPIs as molecular linkers to demonstrate addressable co-immobilization of two enzymes, formate dehydrogenase and lactate dehydrogenase, spatially immobilized on gold and Ni-NTA activated AAO-membrane in a flow reactor. The work highlights the utility of GEPI as genetic linker and molecular erector for addressable immobilization of multiple enzymes potentially useful in variety of technological implementations.
3:50 PM Break
4:10 PM Keynote
Solution Plasma Materials Processing from Natural Products: Nagahiro Saito1; 1Nagoya University
Solution Plasma (SP) is one of non-thermal plasma in liquid phase providing new materials synthesis fields. Various materials can be created by the combination of solvents and solutes. To retain SP states, solution must supply electrons to SP and plasma must supply electric field to solution. These exchanges provide us a unique reaction field. We have been successful to fabricate Au, Pt, AuPt, MnO2 nanoparticles, Pt and AuPt on nanocarbons from aqueous solutions. Recently, we fabricated nanocarbon including heterocarbon from the organic solutions. In this synthesis, various chemicals can be selected as solution, i.e., solvents and solutes. For examples, anthocyanin, which is main component of red wine, is one of starting materials for solution plasma. By using SP, we synthesized hetero-carbon sheets and particles and low-weight molecules of natural products and its composite materials. The synthesis and properties of novel synthesized materials from natural products were reported in this presentation.
Engineering Lactate Oxidases with Metal Binding Peptides towards Lactate Monitoring: Erkan Mozioglu1; Dwight O'Dell1; Thomas Brandon Richard1; Mark L. Richter1; Candan Tamerler1; 1The University of Kansas
Lactate monitoring is of great interest in diverse areas including biomedical such as for diagnosis of diabetes, renal failures, tissue hypoxia or liver failures as well as food industry for beverage production through fermentation. Variety of methods has been developed for sensing and monitoring of lactate in versatile media. Due to their specificity, enzyme-based biosensors have expanding interest. Immobilization of lactate oxidases onto an electrode surface offers to measure the amount of lactate using electrochemical changes. Despite of the variety of techniques developed for enzyme immobilization, enzymatic activity has been limited on the surfaces due to lack of orientation control or harsh chemical environment applied for the surface modification. Here, we engineered a lactate oxidase enzyme to incorporate a metal binding peptide to facilitate the self-assembly of the enzyme onto the electrode surface. Single step self-immobilization of the enzyme resulted in a desirable enzymatic activity on the electrode surface.