3D Printing of Biomaterials and Devices: Session III
Sponsored by: ACerS Bioceramic Division
Program Organizers: Sahar Vahabzadeh, Northern Illinois University; Susmita Bose, Washington State University; Amit Bandyopadhyay, Washington State University; Mukesh Kumar, LincoTek Medical; Mangal Roy, Indian Institute of Technology - Kharagpur (IIT-Kgp)
Tuesday 8:00 AM
October 11, 2022
Room: 319
Location: David L. Lawrence Convention Center
Session Chair: Kalpana Katti, North Dakota State University
8:00 AM Invited
Interlocked Bone Scaffolds with BMP Induced Osteogenesis with Use of 3D Printed Molds: Kalpana Katti1; Krishna Kundu1; Sharad Jaswandkar1; Dinesh Katti1; 1North Dakota State University
An estimated four million bone grafting procedures are performed worldwide each year. Currently, therapies for non-union bone defects are limited. We report development of an interlocked BMP2 and BMP7 coated scaffold block-assembly using 3D printed molds. The interlocked block-assembly maintains high surface area while maintaining the scaffold's structural integrity. The BMPs coated scaffold blocks exhibit increased bone extracellular matrix (ECM) formation. Extended time (9-week) study on in-vitro bone regeneration using the BMPs coated interlocking scaffold blocks showed up to 120% increase in the elastic modulus indicating enhanced ECM formation. We report significant increase in the bone-related protein and osteogenesis related Wnt-factors in BMP coated scaffolds. The BMPs influence ECM formation much longer than the availability of the BMPs in culture-media suggesting that BMPs play a crucial role on initial stages of osteogenesis. Modular and manufacturable BMPs coated interlocking scaffolds made using 3D printed molds provides a novel bone regeneration system.
8:30 AM Invited
Sheet Lamination Additive Manufacturing (SLAM) – A Viable Approach to Resorbable 3D Constructs for Bone Tissue Engineering: Prashant Kumta1; Matthew Criado1; Abhijit Roy1; John Ohodnicki1; Nick Tondravi1; Hannah Fischer1; Howard Kuhn1; 1University of Pittsburgh
Additive manufacturing (AM) has been extensively studied for generating patient specific 3D constructs of bioresorbable magnesium (Mg) alloys for orthopedic applications. Reactivity of Mg and presence of the inherent oxide limits AM of Mg yielding constructs with inferior resolution, mechanical, and materials properties. An AM method exploiting sheet lamination and additive manufacturing (SLAM) offers a creative solution wherein the sheet feedstock obviates powder use and the consequent limitations of powder based conventional AM. This presentation will discuss the SLAM process parameters of temperature, pressure, time, and more importantly, surface roughness tailored to minimize geometric deformation of engineered pores. Results reveal surface finish as a key parameter for generating mechanically stable Mg constructs with control of pore size, shape, and resolution. Materials-process relationships and in-vitro cytocompatibility results will be discussed demonstrating SLAM as a unique AM approach to fabrication of highly advanced high-resolution 3D constructs of Mg and Mg alloys.
9:00 AM Invited
Implant Optimization Guided by Biomimetic Insight: Malcolm Snead1; 1University of Southern California
Implants use has expanded and with it,the prevalence of peri-implant disease that shortens implant life and leads to failure. Peri-implant disease results from microbiota dysbiogenesis triggering a host immune inflammatory response that destroys tissue. At an incidence of 14.5%, and over 3 million implants placed, growing by 500,000/year, reduced service life ending in failure will adversely impact public health and increase health care costs. We developed an antimicrobial bifunctional peptide film to slow disease progression. Based on a high-affinity titanium binding peptide that anchors the anti-microbial peptide to the implant surface, greater than 98% coverage is achieved in <2 minutes even in the presence of contaminating protein, to produce a film durable to mechanical brushing that kills >90% of bacterial. This non-surgical approach improves oral health by delivering a simple to apply/reapply multiple times using a water-delivered bifunctional peptide film serving to control microbial dysbiogenesis and reduce disease progression.
9:30 AM Invited
3D Printing Strategies to Fabricate Complex Scaffolds for Tissue Engineering Applications: Murat Guvendiren1; Alperen Abaci1; 1New Jersey Institute of Technology
3D printing has become a common manufacturing technique to create tissue engineering scaffolds and live tissues. In this invited talk, novel strategies developed in our research laboratory will be discussed to fabricate complex devices composed of hydrogels or solid polymers for tissue engineering applications such as regenerating vasculature and bone tissue. First, we will present a novel approach to bioprinting macro-channels within cell-laden hydrogels to regenerate vasculature. Then, we will focus on bioprinting of cell-laden hydrogels and cell only aggregates within functional and/or support hydrogels, and summarize our results to control stem cell osteogenesis using these devices. We will then focus on controlling stem cell hetereogenity within 3D printed solid polymeric scaffolds with airbrushed membranes for interface tissue engineering.
10:00 AM Break
10:20 AM
Mechanical and Electrical Properties of 3D Printed Wearable Structures: Jose Gonzalez-Garcia1; Bhargavi Mummareddy1; Gina Morrison1; Vamsi Borra1; Pedro Cortes1; Byung-Wook Park1; 1Youngstown State University
Nowadays, wearable sensors emerging from 3D printing technologies are becoming the next generation of integrate platforms for different applications in the health field such as sweat sensing and tactile sensing among others. However, more studies about their mechanical and electrical properties need to be addressed in order to evaluate their response under fatigue conditions over long periods of time. This with the purpose of evaluate their integrity. Indeed, modifications in their manufacturing process including different conductive inks polymeric substrates will affect their performance. Therefore, the aim of this study is to evaluate the structural and electrical properties of 3D printed wearable component where the ink conductivity, as well as the ink-substrate interaction are evaluated. This study will provide the fundamental understanding of flexible printed structures to act as supporting platform for a cortisol sensor system being investigated.
10:40 AM
Effect of Printing Parameters on 3D-printed Biodegradable Biopolymer-metal Composite Material: Prashant Kumta1; John Ohodnicki1; Abhijit Roy1; Matthew Criado1; Howard Kuhn1; 1University of Pittsburgh
Additive manufacturing (AM) has received widespread attention for generating 3D biodegradable scaffolds for tissue engineering comprising biodegradable metals. However, the highly reactive oxide layer present on the surface of the biodegradable magnesium pose many challenges resulting in 3D printed constructs with suboptimal mechanical and materials properties. This presentation addresses this issue using fused deposition modeling (FDM) printing of a novel bio-polymer and bio-metal containing composite material. The effect of printing temperature, layer height, and bio-polymer/bio-metal ratio on the mechanical properties, materials properties, and printed geometrical accuracy and reproducibility of the printed constructs was assessed. In-vitro cytocompatibility studies using MC3T3 pre-osteoblast as well as a pilot in-vivo rat calvarial defect model were also conducted. The presentation will present results from this study showcasing the importance and major influence of the various printing parameters on printing quality, reproducibility, and mechanical properties of the resultant 3D printed composite.