| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biomaterial Applications
|
| Presentation Title |
Platinum Wire-based Aptasensors Exploiting Self-assembled Monolayer (SAM) Components for Cardiac Biomarker Detection |
| Author(s) |
Prashant Nagesh Kumta, Mitali Patil, Sangeetha Kunjukunju, John Ohodnicki |
| On-Site Speaker (Planned) |
Sangeetha Kunjukunju |
| Abstract Scope |
Cardiovascular diseases (CVDs) are the leading cause of death, impacting 92.1 million Americans. CVDs are clinically silent until complications arise and thus are undetected or misdiagnosed. While impedimetric biosensors demonstrate great potential for rapid screening and early detection, optimizing the self-assembled monolayer for tethering the biological detection element to avoid non-specific interference is essential. This research presents optimization of the biosensing platform using aptamer-functionalized platinum wires and electrochemical impedance spectroscopy for detecting two Troponin T and Brain Natriuretic Peptide biomarkers vital for screening myocardial infarction and congestive heart failure conditions. Optimal incubation times, concentrations, and functionalization layer combinations required for biosensing of biomarkers were assessed. Results demonstrate the significance of each functionalized layer, optimal time and concentration of each layer, and the role of each layer in mitigating non-specific interference, thus illustrating the universal potential of this biosensor for rapid screening of CVD and other diseases. |