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Meeting 2017 TMS Annual Meeting & Exhibition
Symposium Characterization of Materials through High Resolution Coherent Imaging
Presentation Title Biological Imaging Using Combined Ptychography and X-ray Fluorescence
Author(s) Karolina Stachnik, Martin Warmer, Pawel Wrobel, Felix Marschall, Istvan Mohacsi, Pontus Fischer, Ismo Vartiainen, Christian David, Marek Lankosz, Alke Meents
On-Site Speaker (Planned) Karolina Stachnik
Abstract Scope Pathogenesis of many diseases is initiated by a number of cellular processes which are unquestionably affected by minor and trace elements, mainly metals. Studies of their contribution require knowledge of their spatial distributions within structures of biological tissue in relation to its (sub-)cellular morphology. Consolidation of ptychographic CDI and nanoscale X-ray fluorescence yields simultaneously complementary information on morphology and elemental distribution of investigated specimen. It therefore facilitates correlative study of radiation-sensitive biomedical samples. We present the application of simultaneous ptychography and nano-XRF of biological tissue sections and cells using fly-scan acquisition mode at beamline P11 at the PETRA III, DESY, Germany. With photon energies up to 8.5 keV, we could access XRF signal from majority of first-row transition metals, obtaining ptychographic reconstructions and corresponding XRF maps. Resolution of obtained images will be discussed and challenges in high-throughput imaging of weakly phase-shifting biomedical samples will be addressed.
Proceedings Inclusion? Planned: Supplemental Proceedings volume

OTHER PAPERS PLANNED FOR THIS SYMPOSIUM

3D Imaging of High-pressure Induced Deformation Twinning in a Nanocrystal
3D X-ray Imaging of Defect Dynamics in Nanostructured Materials
Anisotropic Growth Patterns in Four Dimensions
Applications of High Resolution Coherent X-Ray Imaging Techniques for Investigating Additively Manufactured Materials
Biological and Bio-inspired Multifunctional Structural Materials
Biological Imaging Using Combined Ptychography and X-ray Fluorescence
Biomimetic CaCO3 Complex Morphologies Studied by Coherent X-ray Diffraction Imaging
Characterizing Evolving Processes through Coupled CDI and Molecular Dynamics Studies
Coherent Diffractive Imaging with Wavelength Spatial Resolution using 13.5nm High Harmonics: Full Field, High-contrast Imaging on a Tabletop
Coherent X-ray Diffraction Measurements of Lattice Distortions Caused by Ion Bombardment
Coherent X-ray Imaging at Future High Brightness Synchrotron Sources
High Resolution Coherent Imaging for Materials
High Speed Tomographic Imaging of Materials during Uniaxial Loading
Imaging Strain Fields by Ptychographic Topography
In-Situ and In-Operando Examination of Structure-Functional Relations in Porous Materials for Energy Conversion and Storage with Nano- and Micro- Synchrotron X-ray Computed Tomography
In-situ Deformation and Damage Assessment in Materials under Dynamic Loading Using High Speed Synchrotron X-ray Phase Contrast Imaging
In-situ Phase Contrast Nano-tomography at ID16B
Nanoscale 4D Microstructural Evolution of Precipitates in Aluminum Alloys Using Transmission X-Ray Microscopy (TXM)
Nanoscale Chemical Imaging of an Individual Catalyst Particle with Soft X-ray Ptychography
Phase Contrast Tomography to Document Gypsum Dehydration in Single Crystals and Polycrystalline Materials
Photoelastic Ptychography: A New Approach for Quantitative Stress Determination
Polychromatic Bragg Coherent X-ray Diffraction Imaging for Rapid Measurements
Progress towards Dichroic Bragg Coherent Diffractive Imaging
Real-time Direct and Diffraction Hard X-ray Imaging of Ultra-fast Processes
Revolutions in Coherent X-ray Sources Will Enable Dynamic Nanometer Scale Strain Imaging in Structural Materials
Soft-X-ray Ptychographic Imaging of Shale
Some Recent Advances in the Theory and Modeling of Phase Contrast Imaging
Speckle-based X-ray Imaging at Diamond Light Source
Unraveling the Structure-function Relationships in Ion Implanted Nanodiamonds
Zernike Phase Contrast for Hard X-ray Microscopy

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