|About this Abstract
||2017 TMS Annual Meeting & Exhibition
||Characterization of Materials through High Resolution Coherent Imaging
||Biological Imaging Using Combined Ptychography and X-ray Fluorescence
||Karolina Stachnik, Martin Warmer, Pawel Wrobel, Felix Marschall, Istvan Mohacsi, Pontus Fischer, Ismo Vartiainen, Christian David, Marek Lankosz, Alke Meents
|On-Site Speaker (Planned)
Pathogenesis of many diseases is initiated by a number of cellular processes which are unquestionably affected by minor and trace elements, mainly metals. Studies of their contribution require knowledge of their spatial distributions within structures of biological tissue in relation to its (sub-)cellular morphology. Consolidation of ptychographic CDI and nanoscale X-ray fluorescence yields simultaneously complementary information on morphology and elemental distribution of investigated specimen. It therefore facilitates correlative study of radiation-sensitive biomedical samples. We present the application of simultaneous ptychography and nano-XRF of biological tissue sections and cells using fly-scan acquisition mode at beamline P11 at the PETRA III, DESY, Germany. With photon energies up to 8.5 keV, we could access XRF signal from majority of first-row transition metals, obtaining ptychographic reconstructions and corresponding XRF maps. Resolution of obtained images will be discussed and challenges in high-throughput imaging of weakly phase-shifting biomedical samples will be addressed.
||Planned: Supplemental Proceedings volume