|About this Abstract
||2015 TMS Annual Meeting & Exhibition
||Biological Materials Science Symposium
||F32: Dynamic Modeling Approach to Follow Adsorption Kinetics of Engineered Proteins that Self-assembles Through Biomolecular Recognition
||James Meyer, Viraj Singh, Banu Taktak Karaca, Paulette Spencer, Anil Misra, Candan Tamerler
|On-Site Speaker (Planned)
The intrinsic ability of proteins to interact with inorganics has inspired widespread interest into the biological assembly and patterning of solid materials. Lately many inorganic binding peptides were biocombinatorially selected and then utilized in diverse applications requiring oriented assembly of functional peptides and/or proteins. Many studies focused on the applications; however, their adsorption kinetics dictates their wide-spread utilization. The modified Langmuir adsorption models have proven useful, to some degree, to derive kinetics related parameters. As our understanding of molecular recognition based biomolecular interactions with solid materials grows, establishing an adaptable kinetics model to follow individual binding steps relevant to their bio-self-assembly process becomes a necessity. We developed an integrative computational approach to analyze experimental protein adsorption studied by quartz-crystal-microbalance spectroscopy with dissipation. The model was tested on genetically engineered red fluorescence protein with gold binding ability to follow several distinct regions that may be relevant to different surface binding events.
||Planned: A print-only volume