| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biomaterial Applications
|
| Presentation Title |
Development of a Microfluidic Blood-Vessel-on-Chip Model |
| Author(s) |
Shaurya Prakash |
| On-Site Speaker (Planned) |
Shaurya Prakash |
| Abstract Scope |
We have developed a 3D microfluidic model of a bifurcating vessel to evaluate the effect of electric fields (EF) on regulating endothelial permeability when co-applied with physiologically relevant fluid forces. The microfluidic vessel model also presents a capillary-vessel-soft tissue interface by using a collagen chamber. Therefore, this vessel model captures several key features of an in vivo capillary. In this presentation, I will report on the application of a EF simultaneously with flow to observe a 4-fold increase in endothelial permeability compared to the static control. Using EF to control endothelial permeability provides a potential new way to manipulate angiogenic responses for use in advanced wound therapies. Moreover, the observed increase in endothelial permeability due to combined EF and flow was transient and dependent on the Akt signaling. Collectively, these findings provide significant new insights into how the endothelium serves as an electro-mechanical interface for regulating vessel permeability. |