| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biomaterial Applications
|
| Presentation Title |
Development of Tissue-Specific, Perfusable Vasculature in Microphysiological Systems |
| Author(s) |
Kevin Ling, Arvind Srivatsava, Kannan Mannian, James McGrath, Ruchira Singh, Danielle S.W. Benoit |
| On-Site Speaker (Planned) |
Kevin Ling |
| Abstract Scope |
Advances in tissue engineering and tissue chip technology have catalyzed the rise of microphysiological systems (MPS) as an alternative to animal models for preclinical testing. MPS for vascularized tissues either forego perfused microvasculature or rely on oversimplified endothelial cell-lined fluidic channels. Microvascular tissues are 3D and have tissue specific molecular transport, angioarchitectural, and paracrine-tissue crosstalk properties. Previously, we developed an engineered extracellular matrix (eECM) comprised of poly(ethylene glycol) (PEG) hydrogels crosslinked with matrix metalloproteinase (MMP)-degradable peptides and functionalized with Arginine-Glycine-Aspartic Acid (RGD) cell adhesive ligands that supports vasculogenesis. We introduced this vasculogenic eECM into a novel MPS designed for microvascular network development. Specifically, we explored pressure/flow and eECM biophysical and biochemical cues to guide the development of in vivo-like perfusable microvasculature specific to bone, salivary gland, and the retina. We validated tissue specific angioarchitecture, perfusion, and paracrine crosstalk using immunohistochemistry, microsphere tracking, and western blot analysis. |