Pathogenic mycobacteria have been found with alarming frequency in water distribution networks, hospital water systems, and domestic showerheads. These biofilms are more robust to traditional disinfectants than planktonic bacteria, and they pose a significant risk of infection to immunocompromized patients. Heavy metal nanoparticles, such as silver and copper, have been used in antibacterial coatings on medical implants, textiles, and consumer appliances. The antibacterial effects of silver nanoparticles on planktonic bacteria are well-described in the literature. However, the effect of silver on the more resistant biofilm state is often overlooked. In this research an antibacterial coating of silver nanoparticles was deposited on a uniform surface and the attachment and growth of Mycobacterium smegmatis was observed in a parallel channel bioreactor flow cell. The stages of biofilm development on a silver surface and a negative control were compared with digital optical imaging, SEM, and viable bacteria counting for the first time.