|About this Abstract
||Materials Science & Technology 2013
||Optical and X-ray Imaging Techniques for Material Characterization
||A Simple Laser Ti: Sapphire System Upgrade for Multimodal Nonlinear Optical Microscopy
||Mathieu Laliberté, Antony Bertrand-Grenier, Youngjae Kim, Bryan Burgoyne, Alain Villeneuve, Francois Legare
|On-Site Speaker (Planned)
Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free imaging technique that is capable of real-time, non-pertubative examination of living cells and organisms based on molecular vibrational spectroscopy with a sub-micron spatial resolution. For many applications, CARS microscopy is used with other nonlinear imaging techniques, like 2-photon fluorescence (2PF) and second harmonic generation (SHG). These techniques are typically using a femtosecond laser oscillator for imaging, in contrast with the picosecond optical parametric oscillator (OPO) used for CARS microscopy. Considering that multimodality is the key to image tissues, we have built a laser system to make multimodal nonlinear microscopy, consisting of a femtosecond laser oscillator synchronized electronically with a picosecond laser. Recent results show that this system offers superior stability to the system using two synchronized picosecond Ti-Sa oscillators, and equivalent to using picosecond parametric oscillator, in a system friendly to use for a fraction of the cost.
||Definite: A CD-only volume