Beta titanium alloys attract attentions due to their high strength, ductility, excellent corrosion resistance, and biocompatibility. In this study, the influence of temperature and thermomechanical loading on the strength, hardness, and phase transformation behavior were studied for Ti-13Cr-1Fe-3Al (wt.%), which is a metastable beta titanium alloy. Tensile tests were performed inside a scanning electron microscope to understand the evolution of the deformation behavior from room temperature to 600 C. Slip trace analysis was conducted by using the Electron Backscatter Diffraction data, collected before and after the tests. The Vickers’s hardness was measured before and after the deformation. The results show that ultimate tensile strength, hardness, and deformation modes strongly depend on the temperature and the thermomechanical processes, which influenced the formation of the alpha and other phases. Similar experiments conducted on Timetal21S will also be presented and discussed.