About this Abstract |
Meeting |
2022 Annual International Solid Freeform Fabrication Symposium (SFF Symp 2022)
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Symposium
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2022 Annual International Solid Freeform Fabrication Symposium (SFF Symp 2022)
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Presentation Title |
Characterizing Hydrodynamic Shear Stress and Cell Viability of Algal Cells during Extrusion Bioprinting |
Author(s) |
Keegan Ocorr, Ellis Williams, Srikanthan Ramesh, Ke Du, Iris Rivero, Sara Nafar |
On-Site Speaker (Planned) |
Keegan Ocorr |
Abstract Scope |
The encapsulation of algal cells into a soft material and deposition into 3D structures has numerous applications, such as improving tissue oxygenation, producing metabolites, and aiding in the forming synthetic coral reefs. Protecting the algae contained in the hydrogels from the shear stresses experienced during extrusion remains an obstacle to maximizing the functionality of the printed structures. This research evaluates the influence of shear stress on the viability and function of the encapsulated algal cells. We use ANSYS-Fluent to simulate the flow of algae-laden hydrogels through printer nozzles and determine the shear stress range that allows for continued algae growth and function. A 2D axisymmetric model was used to simulate the flow of cell hydrogels through conical nozzles, and numerical predictions were empirically validated. Our results suggest that optimization of the printing process must be carried while remaining within the maximum limit of shear stress that the cells can handle. |
Proceedings Inclusion? |
Definite: Post-meeting proceedings |