This work has like object to improve the mechanical, tribological, biocompatibility and biofunctional properties of AISI-316, using a monolayer of diamond-like carbon (DLC) doped within W and Si. These hard coatings were studied to improve the strength and biocompatibility of the material and can be applied to the prosthesis, as the head of the femur or knee. The technique used for the growth of the coatings was by RF sputtering. The monolayer were developed using the materials: coal, titanium, silicon and tungsten. It evaluated the biocompatibility of the monolayer by means proof of cell viability, itself involve linfocitos's cultivation; getting experimental moral values good for each monolayer: W-DLC presented a yield of 51,19% over cell viability than Fe (negative control), Si-DLC presented a yield of 48,68% more cell viability in relation to the same control.