|About this Abstract
||2017 TMS Annual Meeting & Exhibition
||Bio-Nano Interfaces and Engineering Applications
||Engineering Lactate Oxidases with Metal Binding Peptides towards Lactate Monitoring
||Erkan Mozioglu, Dwight O'Dell, Thomas Brandon Richard, Mark L. Richter, Candan Tamerler
|On-Site Speaker (Planned)
Lactate monitoring is of great interest in diverse areas including biomedical such as for diagnosis of diabetes, renal failures, tissue hypoxia or liver failures as well as food industry for beverage production through fermentation. Variety of methods has been developed for sensing and monitoring of lactate in versatile media. Due to their specificity, enzyme-based biosensors have expanding interest. Immobilization of lactate oxidases onto an electrode surface offers to measure the amount of lactate using electrochemical changes. Despite of the variety of techniques developed for enzyme immobilization, enzymatic activity has been limited on the surfaces due to lack of orientation control or harsh chemical environment applied for the surface modification. Here, we engineered a lactate oxidase enzyme to incorporate a metal binding peptide to facilitate the self-assembly of the enzyme onto the electrode surface. Single step self-immobilization of the enzyme resulted in a desirable enzymatic activity on the electrode surface.
||Planned: Supplemental Proceedings volume