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Meeting 2018 TMS Annual Meeting & Exhibition
Symposium Bio-nano Interfaces and Engineering Applications Symposium
Presentation Title Self-assembled Formate Dehydrogenase-metal Nanoparticle Hybrids Improved Enzyme Stability
Author(s) Rachel Lietz, Sarah VanOosten, Erkan Mozioglu, Brandon Tomas, Kasra Alizadeh, Mark Richter, Candan Tamerler
On-Site Speaker (Planned) Rachel Lietz
Abstract Scope Formate dehydrogenase (FDH) plays a critical role in regenerating NADH and producing methanol and formic acid, which can be used as precursors to novel fuels and chemicals. Despite its great potential, FDH has limited industrial use due to its low stability and lack of reusability thus there is significant interest in immobilization of this enzyme. Current methods involves mainly covalent immobilization of enzymes, which jeopardize enzymatic function by altering its optimum configuration in addition to involving harsh chemicals. Here we utilize a metal binding peptide (MtBP) to functionalize formate dehydrogenase (FDH) onto the surface of gold nanoparticles. We verified binding and enzyme activity on the gold nanoparticles using L-SPR and UV-Vis spectroscopic analyses, respectively. Finally enzymatic stability was monitored over a range of temperature and pH values. The engineered FDH with peptide tag could be used as a robust biocatalyst system for biomedical and energy applications.
Proceedings Inclusion? Planned: Publication Outside of TMS (Indicate publication title and publisher if known.)

OTHER PAPERS PLANNED FOR THIS SYMPOSIUM

Bioconjugated Nanoparticle Imaging Probes for Molecular Imaging with Computed Tomography
Biofilm Formation Behavior on Polymer Brush Surfaces by E.coli and S. Epidermidis
Biomolecular Design of Soft Interfaces for Technology and Medicine
Chemically Functionalised Graphene FET with Double Conductance Minima for the Label-free Sensing of Exosomes
Computational Study of Selective Adsorption of Peptides on MoS2 Surface
Connecting Biology and Electronics with Protons: From Ion Channels to Cells
Development of FRET Biosensor Based on Aptamer/Functionalized Graphene for Ultrasensitive Detection of Bisphenol A and Discrimination from Analogues
Discovery of Biomaterials by Simulation and Experiment: Catalysts, Composites, and Therapeutics
Effects of Hydration and Mineralization on the Mechanical Behavior of Collagen Fibrils
Investigating the Interaction of Amyloidogenic Proteins with Inorganic Surfaces, Nanoparticles and Biomolecules by Atomistic Simulations
Modeling the Mechanics of Cancer Cells on Tissue Engineering Substrates
Nanoclay Based Tissue Engineering Scaffolds for Mimicking Biointerfaces in Mesenchymal to Epithelial Transision of Prostate and Breast Cancer Metastasis to Bone
Nanoparticles-grafted Functionalized Graphene Coated with Nanostructured Polyaniline Layered Nanocomposites for High-performance Biosensors
Nanoscale Structure and Properties of Biomaterials
New Antimicrobial Peptides Generated through Genetic Algorithm Approach Using Chemical Property Based Cross-over 
Oligopeptides and Recombinamers at Surfaces and Interfaces to Address Oral Infections
Revealing the Full Hierarchical Structure of Spider Silks across All Length Scales
Revealing the Multi-functional Surface and Material Property of Venus Flytrap (Dionaea Muscipula)
Self-assembled Formate Dehydrogenase-metal Nanoparticle Hybrids Improved Enzyme Stability
Single Molecular Imaging of Fluorescent-tagged Peptides Diffusing on a Surface of Boron Nitride
Smart Biomaterials for MoS2 and Gold Mining
Surface Modification of Ti6Al4V to Confer Antibacterial Properties against Listeria Monocytogenes
Synthesis and Characterization of Superomniphobic Surfaces Inspired by Springtails
Transmission Synchrotron X-ray Tomography and Nano-indentation Measurements for the Investigation of the Teeth Microstructure of Dinosaurs
Transparent Titanium Dioxide Nanotubes: Processing, Characterization, and Application in Establishing Cellular Response Mechanisms
Use of Nanostructured Diamond in Medical Device Applications

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