|About this Abstract
||Materials Science & Technology 2011
||Emerging Frontiers in Surface Engineering of Biomaterials
||The Influence of Topographic Features Produced by Laser Micromachining and Surface Diffusion Techniques on Bone Cell Behavior
||Jorge Sobral, William O'Neill, Trevor Clyne
|On-Site Speaker (Planned)
There is a lot of interest in new methods to improve the proliferation and adhesion of bone cells to the surface of prosthetic implants. In this study several surface topographies were produced by laser processing and heat treatment techniques on stainless steel specimens in order to evaluate their influence on bone cell behavior, in vitro. Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and X-Ray Photoelectron Spectroscopy (XPS) techniques were used to characterize the such topographic features. Human fetal osteoblasts (hFOB) were seeded on such specimens and AlamarBlue, DNA assays, SEM imaging, Alkaline phosphatase and immunochemistry assays were used to fully describe cell behavior. The results suggest that using these techniques it is possible to create a wide range of topographies at micro and nano scale that are able to control cell behavior and this might be a feasible way of increasing the potential of metals for biomedical applications.
||Definite: A CD-only volume