Eletrochemical behavior and corrosion properties of 3D printing Ti-6Al-4V alloy in artificial seawater at temperature ranges from 20℃to 40℃ were investigated using electrochemical techniques, including potentiodynamic polarization, electrochemical impedance spectroscopy (EIS), and Mott-Schottky measurement, etc. Equivalent circuits were simulated to investigate alloy surface and analyse effects of temperature on interface of alloy and solution. The results show that corrosion potential increased with temperature rise, and the EIS presented a single capacitive resistance characteristics. When the temperature reached 35℃ or more, passive current increased, the passivation phenomenon was not obvious, and corresponding radiating arc radius reduced, indicating that the resistance decreased, conductivity of surface passivation film improved, and corrosion performance reduced. According to the Mott-Schottky curves, alloys exhibited n-type semiconductor characteristics. With increasing temperature, conductivity of passivation film improved, and alloy corrosion resistance decreased. At 40℃, alloy possessed the worst corrosion resistance.