|About this Abstract
||Materials Science & Technology 2011
||Next Generation Biomaterials
||Application of Polymer-Based Microfluidic Devices for the Selection and Manipulation of Low-Abundant Biological Cells
||Malgorzata A Witek, Udara Dharmasiri, Samuel Njoroge, Morayo G. Adebiyi, Joyce Kamande, Mateusz L Hupert, Francis Barany, Steven A Soper
|On-Site Speaker (Planned)
||Malgorzata A Witek
Microfluidics are providing new opportunities for diagnostics. Innovative technologies developed in our group allow for the selection, enumeration, and molecular profiling of low-abundance (<100 cells/mL) circulating tumor cells (CTCs) directly from blood, a recognized assay for the management of cancer-related diseases. Colorectal cancer cells overexpressing the integral membrane protein, EpCAM, were immunospecifically isolated using anti-EpCAM antibodies immobilized to the walls of a selection bed poised on a microfluidic device that could process >1 mL. The cells were enzymatically released from the selection surface, and hydrodynamically transported for conductivity-based enumeration through an electrodes pair. Following counting, CTCs were electrophoretically withdrawn from the bulk flow and preconcentrated into an anodic reservoir. The molecular processing invoked a strategy consisting of a primary PCR for amplification of the specific gene fragment, and an allele-specific ligation reaction to detect mutations within gene. The presence/absence of mutations in KRAS could be used to guide patient chemotherapy.