| About this Abstract |
| Meeting |
Materials Science & Technology 2011
|
| Symposium
|
Next Generation Biomaterials
|
| Presentation Title |
Application of Polymer-Based Microfluidic Devices for the Selection and Manipulation of Low-Abundant Biological Cells |
| Author(s) |
Malgorzata A Witek, Udara Dharmasiri, Samuel Njoroge, Morayo G. Adebiyi, Joyce Kamande, Mateusz L Hupert, Francis Barany, Steven A Soper |
| On-Site Speaker (Planned) |
Malgorzata A Witek |
| Abstract Scope |
Microfluidics are providing new opportunities for diagnostics. Innovative technologies developed in our group allow for the selection, enumeration, and molecular profiling of low-abundance (<100 cells/mL) circulating tumor cells (CTCs) directly from blood, a recognized assay for the management of cancer-related diseases. Colorectal cancer cells overexpressing the integral membrane protein, EpCAM, were immunospecifically isolated using anti-EpCAM antibodies immobilized to the walls of a selection bed poised on a microfluidic device that could process >1 mL. The cells were enzymatically released from the selection surface, and hydrodynamically transported for conductivity-based enumeration through an electrodes pair. Following counting, CTCs were electrophoretically withdrawn from the bulk flow and preconcentrated into an anodic reservoir. The molecular processing invoked a strategy consisting of a primary PCR for amplification of the specific gene fragment, and an allele-specific ligation reaction to detect mutations within gene. The presence/absence of mutations in KRAS could be used to guide patient chemotherapy. |
| Proceedings Inclusion? |
Undecided |