| About this Abstract |
| Meeting |
2020 TMS Annual Meeting & Exhibition
|
| Symposium
|
Advanced Real Time Imaging
|
| Presentation Title |
New Biomarkers of Prostate and Breast Cancer Metastasis to Bone |
| Author(s) |
Kalpana S. Katti, Sumanta Kar, Haneesh Jasuja, MD Shahjahan Molla, Dinesh R Katti |
| On-Site Speaker (Planned) |
Kalpana S. Katti |
| Abstract Scope |
A novel cancer metastasis in vitro model was built using tissue-engineering approaches with nanoclay scaffolds that enable proliferation and differentiation of human mesenchymal cells to form hierarchical structures mimicking remodeling bone. Both prostate and breast cancer have the propensity to metastasize to bone thus causing fatalities. Cancer cell lines are seeded on the newly formed bone like structures. Gene and protein expression studies indicate that the tumors created in vitro are Mesenchymal to Epithelial transition(MET) of cancer metastasis. Nanoindentation is used obtain mechanical response of tumors. Softening of tumor cells at MET as well as softening at metastasis progression is reported. FTIR experiments indicate unique signatures of DNA and protein contents during cancer progression. Immunocytochemistry as well as gene expression studies indicate a vital role of cytoskeletal elements in the mechanics of cancer cells at tumor progression. The cancer testbed enables the discovery of novel markers for metastasis. |
| Proceedings Inclusion? |
Planned: |