|About this Abstract
||2018 TMS Annual Meeting & Exhibition
||Recent Developments in Biological, Structural and Functional Thin Films & Coatings
||Nickel Oxide Thin Film Based Electrode for Cholesterol Monitoring Using Electrochemical Biosensor
||Gurpreet Kaur, Monika Tomar, Vinay Gupta
|On-Site Speaker (Planned)
Electrochemical biosensors propose an attractive alternative to conventional diagnostic techniques because of their high sensitivity, low detection limits, rapid response and possibility of miniaturization into portable handheld devices. Nickel oxide (NiO) thin film is an ideal matrix for electrochemical biosensors owing to its biocompatibility, chemical stability, rapid electron communication feature and presence of Ni2+-Ni3 redox couple. Thus, in the present work, NiO thin film has been sputtered on a conducting platform for immobilization of biomolecules (cholesterol oxidase and esterase enzymes and anti-apolipoprotein B antibody). Deposition conditions have been optimized (100% pure O2 gas ambient and 20 mTorr sputtering pressure) to fabricate NiO thin film exhibiting nanostructured morphology with fast electron communication feature. Sensitive and selective electrochemical detection of free cholesterol, total cholesterol and low density lipoprotein has been carried out. The biosensor has been miniaturized and integrated with polydimethylsiloxane (PDMS) microchannels demonstrating its ability for point-of-care diagnostics.
||Planned: Supplemental Proceedings volume