|About this Abstract
||2017 TMS Annual Meeting & Exhibition
||Bio-Nano Interfaces and Engineering Applications
||Characteristics of von Willebrand Factor Adhesion on Collagen Surface under Flow
||Wei Wei, Chuqiao Dong, Michael Morabito, Xiaohui Zhang, Wei Zhang, Yan Xu, Wenli Ouyang, xuanhong cheng, Edmund Webb, Alparslan Oztekin
|On-Site Speaker (Planned)
In injured arteries, fascinating bio-nano interfacial phenomena occur during blood clotting that are facilitated by the multimeric protein, von Willebrand Factor (VWF). In normal blood flow, VWF adopts globule conformations; near injury sites, hydrodynamic force from increased flow elongates VWF, exposing binding sites for collagen on the injured arterial surface. Herein we present results from molecular simulations - using parameters from single molecule force spectroscopy experiments - to study binding between VWF and collagen sites. Bond formation and rupture were described via Bell model kinetics and Brownian dynamics simulations were performed to understand VWF multimer adhesion in varying flow conditions. By implementing a binding condition dependent on molecular conformation local to the model VWF binding site, model adhesion predictions were brought into agreement with experimental observations, which show increased adhesion at higher flow rate. Molecular structure at the interface will be presented to help elucidate mechanisms governing VWF functionality.
||Planned: Supplemental Proceedings volume