|About this Abstract
||Materials Science & Technology 2012
||Surface Properties of Biomaterials III
||Calcium Phosphate Composite Coating for Surface Mediated Non-Viral Gene Transfer
||Subhadip Bodhak, Masanori Kikuchi, Ayako Oyane, Yu Sogo, Hideo Tsurushima, Atsuo Ito
|On-Site Speaker (Planned)
The objective of our research is to develop an efficient surface mediated non viral gene transfer system through the immobilization of plasmid DNA in calcium phosphate (CaP) composite coatings. Our research group has successfully fabricated plasmid DNA immobilized calcium phosphate coating on bioinert polymer surfaces through a biomimetic process using supersaturated solutions. Interestingly, it is found that DNA immobilization as well as the gene transfection efficiency of calcium phosphate coatings can be significantly enhanced in presence of lipids and cell adhesive proteins into the composite coating microstructure. Further, we fabricated DNA immobilized CaP coatings on bone-like self-organized hydroxyapatite and collagen (HAp/Col) nanocomposite substrate for bone tissue regeneration applications. The resulting DNA immobilized CaP coated HAp/Col nanocomposites are endowed with combined benefits of osteoconductive surface and gene transfection capabilities providing a functionalized highly bioactive surface to support cell adhesion and growth by stimulating the cells effectively via surface-mediated gene transfer.
||Planned: None Selected