|About this Abstract
||2018 TMS Annual Meeting & Exhibition
||Bio-nano Interfaces and Engineering Applications Symposium
||Transparent Titanium Dioxide Nanotubes: Processing, Characterization, and Application in Establishing Cellular Response Mechanisms
||Jevin Meyerink, Divya Kota, Scott T. Wood, Grant A. Crawford
|On-Site Speaker (Planned)
Titanium dioxide (TiO2) nanotubes have been shown to strongly enhance bone cell behavior and, consequently, have gained attention as potential osteogenetic surface treatments for titanium-bone implants. The exact mechanism by which TiO2 nanotubes influence cellular function remains controversial, partly due to limitations in existing cellular imaging methods with opaque substrates. Through the fabrication and implementation of transparent TiO2 nanotube imaging platforms, this work aims to establish the mechanistic relationship between nanostructure and bone cell responses to ultimately inform the development of advanced osteogenetic surface treatments for titanium-bone implants. This work identifies fabrication conditions for successful production of highly transparent TiO2 nanotube arrays with tailorable diameters, as well as their functionality with biological cells. Pre-osteoblast mouse cells (MC3T3-E1) containing fluorescently-tagged focal adhesion protein vinculin and cytoskeletal filament actin were implemented to record real-time, cell-substrate interaction mechanisms via conventional confocal fluorescent microscopy and fluorescent lifetime imaging (FLIM).
||Planned: Publication Outside of TMS (Indicate publication title and publisher if known.)