|About this Abstract
||2016 TMS Annual Meeting & Exhibition
||Bio Nano Interfaces and Engineering Applications
||D-3: Selection of Peptide Aptamer with Ultrahigh Affinity for TiO2 by Combination of Phage Display and Electroporation
||Ippei Inoue, Yasuaki Ishikawa, Yukiharu Uraoka, Ichiro Yamashita, Hisashi Yasueda
|On-Site Speaker (Planned)
Peptide aptamers with specific affinity for inorganic target substrates have been widely used in construction of hybrid bio-inorganic interfaces. In general, screening of peptide aptamer has been performed using M13 phage display method. Although many aptamers have been obtained by the method, it was difficult to recover the intact phage bodies from the rigid complexes between the substrate and aptamers in the phages. To overcome the problem, we have tried to electrically transfer phage-DNA into Escherichia coli cells using a “direct” electroporation with the phage-substrate complexes. As a result, a novel peptide aptamer (named ST1) with higher affinity for TiO2 particles was selected. A cage-shaped protein fused with both ST1 and CNT-affinity peptide was designed and the protein could be efficiently used for construction of TiO2-CNT nano-hybrid which increased a solar cell performance. This combination method is a simple and rapid screening for aptamers with super-high affinity for target substrates.
||Planned: Publication Outside of TMS (Indicate publication title and publisher if known.)