|About this Abstract
||2017 TMS Annual Meeting & Exhibition
||Biological Materials Science
||Nano- and Micro- scale Mechanical Properties of the Sclera following Proteoglycan Degradation
||Zhuola Zhuola, Riaz Akhtar, Zhuo Chang
|On-Site Speaker (Planned)
The mechanical properties of ocular tissues such as the sclera (white of the eye) have a major impact on the healthy function of the eye, and are governed by the properties and composition of the microstructural components. For example, biomechanical degradation associated with myopia occurs alongside a reduction of proteoglycans. Here, the role of proteoglycan degradation on the nano- and micromechanical properties of the porcine sclera is explored. In vitro enzymatic degradation of proteoglycans was conducted with α-amylase solution. Collagen fibril morphology and nanomechanical stiffness was measured with atomic force microscopy (AFM) and micromechanical properties with dynamic nanoindentation. The elastic modulus of the tissue was reduced in all α-amylase treated samples relative to controls. In addition, collagen fibril organisation was disrupted by proteoglycan depletion. Our data demonstrate that proteoglycans play an important role in determining not only the mechanical properties at these length scales but also collagen fibril arrangement.
||Planned: Supplemental Proceedings volume