|About this Abstract
||2018 TMS Annual Meeting & Exhibition
||Bio-nano Interfaces and Engineering Applications Symposium
||Development of FRET Biosensor Based on Aptamer/Functionalized Graphene for Ultrasensitive Detection of Bisphenol A and Discrimination from Analogues
||Sanju Gupta, Rebecca Wood
|On-Site Speaker (Planned)
We report ultrasensitive efficient platform for bisphenol A (BPA) detection based on Förster resonance energy transfer (FRET) between functionalized graphene (graphene oxide; GO and reduced GO; rGO) as acceptor and fluorescently tagged BPA specific aptamer (FAM-ssDNA) donor design. Due to noncovalent assembly, fluorescent quenching of FAM probe takes place due to good overlap between FAM emission and the GO (and rGO) absorption spectrum. On addition of BPA analyte, the aptamer preferentially bounds to BPA, forming FAM-ssDNA–aptamer–BPA complexes, the FRET is disrupted possibly by switching its configuration or re-hybridization preventing aptamer from molecular adsorption on or away from GO or rGO surface and the fluorescence signal is restored (turn-on’ bioassay). The intensity of optical fluorescence signal was changed in linear proportion to BPA concentration with limit of detection ~10 pg/mL. The sensitivity and specificity of biosensor toward BPA was demonstrated via several BPanalogues and successfully implementing for real water samples.
||Planned: Publication Outside of TMS (Indicate publication title and publisher if known.)