| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Society for Biomaterials: Biomaterial Applications
|
| Presentation Title |
Hydroxyapatite Loaded with 2-heptylcyclopropane-1-carboxylic Acid Inhibits S. Aureus Biofilm Formation |
| Author(s) |
Emily Coleman Montgomery, Isabella Bianca Reaņo, Rachel Wiley, Daniel L Baker, J. Amber Jennings |
| On-Site Speaker (Planned) |
Emily Coleman Montgomery |
| Abstract Scope |
Hydroxyapatite is a natural mineral component of tissue and often used in synthetic biomaterials for its bone-like mechanical and chemical properties. Non-antibiotic methods for infection prevention, such as the use of short chain fatty acid signaling molecules like 2-heptylcyclopropane-1-carboxylic acid (2CP), are advantageous. In this study, 2CP was loaded onto hydroxyapatite coupons to assess the effect on microbial planktonic growth and biofilm formation. 2CP was dissolved in ethanol and deposited on hydroxyapatite coupons. After 3 days of immersion in PBS with daily media changes, Staphylococcus aureus was added to coupons and incubated at 37°C for 24h. The coupons were removed and washed three times in sterilized phosphate buffered saline (PBS) before sonication in PBS to detach biofilm. Microbial viability for each group was quantified using Bactiter-Glo viability assay, which revealed a significant reduction in planktonic growth, biofilm on the coupons, and biofilm on the well plates (p<<0.001). |