| About this Abstract |
| Meeting |
MS&T22: Materials Science & Technology
|
| Symposium
|
Additive Manufacturing and Cellular/Lattice Structures: Designs, Realization and Applications
|
| Presentation Title |
A-1: Cytotoxicity of Strontium Calcium Polyphosphate on MC3T3-E1 Cells in 3D Printed Alginate/Collagen Scaffolds |
| Author(s) |
Sally Lee, Serin Ahn, Chris Mathew, Abdulhadi Badran, Shebin Tharakan, Shams Khondkar, Michael Hadjiargyrou, Azhar Ilyas |
| On-Site Speaker (Planned) |
Sally Lee |
| Abstract Scope |
Alginate and collagen are cell-compatible biomaterials that foster spontaneous proliferation. Prior studies have shown strontium calcium polyphosphate (SCPP) as a suitable crystalline compound to enhance bone formation in osteoblasts. Concentrations of 1% SCPP have shown the greatest bone growth compared to higher cytotoxic concentrations. Our study utilizes 1% SCPP in a dose-dependent manner to evaluate cytotoxicity on MC3T3-E1 cells in Alginate/Collagen composites in-vitro. MC3T3-E1 cells were seeded in at 1,000 cells/well with doses of 2 to 20 mg of only 1% SCPP. Cytotoxicity was observed at 20 mg, likely due to a low pH of the SCPP resulting in severe cytotoxicity. We hypothesize 3D bioprinted cell-laden scaffolds will demonstrate greater proliferation and less cytotoxicity due to alginate’s inherent rigid properties and collagen’s cell-binding sites. The results of cell-surface interactions of these scaffolds for cell growth, proliferation, and the mineralized tissue formation in-vitro will also be presented and discussed. |